A yeast artificial chromosome-based map of the region of chromosome 20 containing the diabetes-susceptibility gene, MODY1, and a myeloid leukemia related gene.

Stoffel M, Le Beau MM, Espinosa R, Bohlander SF, Le Paslier D, Cohen D, Xiang KS, Cox NJ, Fajans SS, Bell GI
Proc Natl Acad Sci U S A. 1996 93 (9): 3937-41

PMID: 8632993 · PMCID: PMC39463 · DOI:10.1073/pnas.93.9.3937

We have generated a physical map of human chromosome bands 20q11.2-20q13.1, a region containing a gene involved in the development of one form of early-onset, non-insulin-dependent diabetes mellitus, MODY1, as well as a putative myeloid tumor suppressor gene. The yeast artificial chromosome contig consists of 71 clones onto which 71 markers, including 20 genes, 5 expressed sequence tags, 32 simple tandem repeat DNA polymorphisms, and 14 sequence-tagged sites have been ordered. This region spans about 18 Mb, which represents about 40% of the physical length of 20q. Using this physical map, we have refined the location of MODY1 to a 13-centimorgan interval (approximately equal to 7 Mb) between D20S169 and D20S176. The myeloid tumor suppressor gene was localized to an 18-centimorgan interval (approximately equal to 13 Mb) between RPN2 and D20S17. This physical map will facilitate the isolation of MODY1 and the myeloid tumor suppressor gene.

MeSH Terms (22)

Base Sequence Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 20 Diabetes Mellitus, Type 2 Disease Susceptibility DNA DNA Primers Electrophoresis, Agar Gel Female Genes, Tumor Suppressor Genetic Markers Haplotypes Humans In Situ Hybridization, Fluorescence Leukemia, Myeloid Male Molecular Sequence Data Pedigree Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Sequence Tagged Sites

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