Construction of an alkaline phosphatase fusion-generating transposon, mTn10phoA.

McClain MS, Engleberg NC
Gene. 1996 170 (1): 147-8

PMID: 8621078 · DOI:10.1016/0378-1119(95)00856-x

We constructed a derivative of the mini-transposon mTn10 that generates translational fusions to the phoA gene from Escherichia coli and carries the KmR determinant from Tn5. This new transposon, mTn10phoA, is carried on a mobilizable plasmid with both selectable and counterselectable markers. The plasmid carrying mTn10phoA was introduced into Legionella pneumophila. Southern hybridization analysis indicated that the mTn10phoA insertions were randomly distributed.

MeSH Terms (9)

Alkaline Phosphatase Bacterial Proteins Base Sequence Cloning, Molecular DNA Transposable Elements Escherichia coli Kanamycin Resistance Recombinant Fusion Proteins Selection, Genetic

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