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TAP1-deficient mice select a CD8+ T cell repertoire that displays both diversity and peptide specificity.

Sandberg JK, Chambers BJ, Van Kaer L, Kärre K, Ljunggren HG
Eur J Immunol. 1996 26 (2): 288-93

PMID: 8617293 · DOI:10.1002/eji.1830260203

Mice deficient in the gene encoding the transporter associated with antigen processing 1 (TAP1) are defective in providing major histocompatibility complex (MHC) class I molecules with cytosolic peptides. Consequently, these mice express reduced levels of MHC class I glycoproteins on the cell surface, and have reduced numbers of CD8+ T cells in the periphery. In the present study, we have addressed the diversity and specificity of the peripheral CD8+ T cell population in TAP1 -/- mice. CD8+ T cells were polyclonal with regard to T cell receptor (TCR) V beta expression. Overall, V beta usage in TAP1 -/- mice appear to be very similar to that in wild-type mice, with significantly reduced levels of V beta 5.1/5.2-expressing CD8+ T cells as the only clear exception. This polyclonal population of CD8+ T cells readily mounted epitope-specific CTL responses against four out of five well-defined MHC class I-restricted peptides. In contrast to allospecific CTL, peptide-specific CTL from TAP1 -/- mice did not cross-react on cells expressing normal levels of H-2b class I. The present results demonstrate that a polyclonal CD8+ T cell repertoire, displaying both diversity and peptide specificity, is positively selected in mice devoid of a functional peptide transporter. These observations imply that TAP-dependent peptides are not absolutely required for positive selection of a functionally diverse repertoire of CD8+ T cells.

MeSH Terms (15)

Amino Acid Sequence Animals ATP-Binding Cassette Transporters ATP Binding Cassette Transporter, Subfamily B, Member 2 CD8-Positive T-Lymphocytes Cytotoxicity, Immunologic Dose-Response Relationship, Immunologic Epitopes H-2 Antigens Immunophenotyping Mice Mice, Inbred C57BL Mice, Mutant Strains Molecular Sequence Data T-Lymphocytes, Cytotoxic

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