The Legionella pneumophila hel locus encodes intracellularly induced homologs of heavy-metal ion transporters of Alcaligenes spp.

McClain MS, Hurley MC, Brieland JK, Engleberg NC
Infect Immun. 1996 64 (5): 1532-40

PMID: 8613357 · PMCID: PMC173958

We continued characterization of the Legionella pneumophila hel locus. Mutagenesis and DNA sequencing identified three genes similar to the czc and cnr loci of Alcaligenes eutrophus and the ncc locus of Alcaligenes xylosoxidans. On the basis of their similarity to these loci, we designated the L. pneumophila genes helC, helB, and helA. Mutations in the hel genes led to reduced cytopathicity towards U937 cells, although the mutant strains did not appear defective in other assays of virulence. Transcription of the hel locus was induced by the intracellular environment but was not induced by any of a variety of in vitro stress conditions. The function of the hel gene products remains to be determined.

MeSH Terms (18)

Alcaligenes Amino Acid Sequence Bacterial Proteins Base Sequence Carrier Proteins Cell Line Chromosome Mapping DNA, Bacterial Gene Expression Regulation, Bacterial Genes, Bacterial Humans Ion Transport Legionella pneumophila Metals Molecular Sequence Data Mutation Species Specificity Virulence

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