Live subgroup B respiratory syncytial virus vaccines that are attenuated, genetically stable, and immunogenic in rodents and nonhuman primates.

Crowe JE, Bui PT, Firestone CY, Connors M, Elkins WR, Chanock RM, Murphy BR
J Infect Dis. 1996 173 (4): 829-39

PMID: 8603960 · DOI:10.1093/infdis/173.4.829

Optimal immunization of neonates against disease caused by respiratory syncytial virus (RSV) probably will require multiple doses of a vaccine containing viruses of both subgroups A and B. Live subgroup B RSV mutants were generated containing multiple attenuating mutations, ts (temperature-sensitive) and non-ts (host range), that were introduced by prolonged passage in cell culture or by chemical mutagenesis. The cold-passaged (cp)-52 mutant was restricted in replication compared to wild type virus in rodents and nonhuman primates. In addition, the attenuation phenotype of cp-52 was stable after prolonged replication in immunosuppressed rodents. One or two ts mutations were then introduced into the cp-52 mutant to generate additional candidate vaccine strains that were more attenuated in vivo than the cp-52 parental virus. Tests in humans are being done to determine if one or more of the RSV B-1 mutants exhibit a satisfactory balance between attenuation and immunogenicity.

MeSH Terms (11)

Animals Antibodies, Viral Cercopithecus aethiops Mutagenesis Respiratory Syncytial Viruses Respiratory Syncytial Virus Infections Serotyping Sigmodontinae Vaccines, Attenuated Viral Vaccines Virus Replication

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