The white gene of Ceratitis capitata: a phenotypic marker for germline transformation.

Zwiebel LJ, Saccone G, Zacharopoulou A, Besansky NJ, Favia G, Collins FH, Louis C, Kafatos FC
Science. 1995 270 (5244): 2005-8

PMID: 8533095 · DOI:10.1126/science.270.5244.2005

Reliable germline transformation is required for molecular studies and ultimately for genetic control of economically important insects, such as the Mediterranean fruit fly (medfly) Ceratitis capitata. A prerequisite for the establishment and maintenance of transformant lines is selectable or phenotypically dominant markers. To this end, a complementary DNA clone derived from the medfly white gene was isolated, which showed substantial similarity to white genes in Drosophila melanogaster and other Diptera. It is correlated with a spontaneous mutation causing white eyes in the medfly and can be used to restore partial eye color in transgenic Drosophila carrying a null mutation in the endogenous white gene.

MeSH Terms (19)

Amino Acid Sequence Animals Animals, Genetically Modified ATP-Binding Cassette Transporters Base Sequence Cloning, Molecular Diptera Drosophila melanogaster Drosophila Proteins Eye Color Eye Proteins Genes, Insect Genetic Markers Insect Hormones Molecular Sequence Data Mutation Phenotype Sequence Alignment Transformation, Genetic

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