Schizosaccharomyces pombe skp1+ encodes a protein kinase related to mammalian glycogen synthase kinase 3 and complements a cdc14 cytokinesis mutant.

Plyte SE, Feoktistova A, Burke JD, Woodgett JR, Gould KL
Mol Cell Biol. 1996 16 (1): 179-91

PMID: 8524294 · PMCID: PMC230991 · DOI:10.1128/mcb.16.1.179

We report the cloning of the skp1+ gene, a Schizosaccharomyces pombe homolog of the glycogen synthase kinase 3 (GSK-3) family whose members in higher eukaryotes are involved in cell fate determination, nuclear signalling, and hormonal regulation. skp1 is 67% identical to mammalian GSK-3 beta and displays similar biochemical properties in vitro. Like GSK-3 beta, skp1 is phosphorylated on a conserved tyrosine residue, and this phosphorylation is required for efficient activity. skp1 is also phosphorylated at a serine which has been identified as S-335. Phosphorylation at this site is likely to inhibit its function. Unlike the mammalian enzyme, skp1 both tyrosine autophosphorylates in yeast cells and can phosphorylate other proteins on tyrosine in bacteria. The skp1+ gene is not essential. However, cells with deletions in skp1+ are sensitive to heat shock and exhibit defects in sporulation. Overexpression of wild-type skp1+ specifically complements cdc14-118, one of several mutations causing a defect in cytokinesis. In addition, certain phosphorylation site mutants induce a delay or block in cytokinesis when overexpressed. Together, these data identify novel interactions of a fission yeast GSK-3 homolog with elements of the cytokinesis machinery.

MeSH Terms (23)

Amino Acid Sequence Animals Base Sequence Calcium-Calmodulin-Dependent Protein Kinases Cell Cycle Proteins Cell Division Cloning, Molecular DNA, Complementary DNA, Fungal Fungal Proteins Gene Deletion Genes, Fungal Genetic Complementation Test Glycogen Synthase Kinase 3 Glycogen Synthase Kinases Humans Molecular Sequence Data Mutation Phosphorylation Protein Tyrosine Phosphatases Saccharomyces cerevisiae Proteins Schizosaccharomyces Sequence Homology, Amino Acid

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