Biosynthesis and in vivo localization of the decapentaplegic-Vg-related protein, DVR-6 (bone morphogenetic protein-6).

Wall NA, Blessing M, Wright CV, Hogan BL
J Cell Biol. 1993 120 (2): 493-502

PMID: 8421061 · PMCID: PMC2119526 · DOI:10.1083/jcb.120.2.493

DVR-6 (BMP-6 or Vgr-1) is a member of the TGF-beta superfamily of polypeptide signaling molecules. In situ hybridization studies have previously shown that DVR-6 RNA is expressed in a variety of cell types in the mouse embryo, but no information has been available on protein localization and biosynthesis. We have produced a polyclonal antibody to the proregion of DVR-6 and used it to localize the protein in whole mount and sectioned embryonic, newborn, and adult mouse tissues. DVR-6 protein is expressed in the mouse nervous system beginning at 9.5 days postcoitum (d.p.c.) and continues through adulthood. A variety of epithelial tissues also produce DVR-6 protein, including the suprabasal layer of the skin, bronchiolar epithelium, and the cornea. Additionally, a stably transfected cell line, BMGE+H/D6c4, is used to study the biosynthesis of DVR-6 protein and evidence is presented for translational regulation of DVR-6 expression.

MeSH Terms (21)

Animals Bone Morphogenetic Proteins Cattle Cells, Cultured Embryo, Mammalian Embryonic and Fetal Development Epithelial Cells Epithelium Female Glutathione Transferase Growth Substances Humans Immunohistochemistry Mammary Glands, Animal Mice Nervous System Protein Biosynthesis Proteins Recombinant Fusion Proteins Transcription, Genetic Transfection

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