Intestinal Na+/H+ exchanger activity is up-regulated by bowel resection in the weanling rat.

Sacks AI, Acra SA, Dykes W, Polk DB, Barnard JA, Pietsch J, Ghishan FK
Pediatr Res. 1993 33 (3): 215-20

PMID: 8384709 · DOI:10.1203/00006450-199303000-00001

Increased Na+/H+ exchanger activity is associated with cellular hyperplasia. Cellular hyperplasia is an adaptive response to small-intestinal resection. Therefore, we hypothesized that the small-intestinal Na+/H+ exchanger activity increases in response to small-intestinal resection. Twenty-one-d-old, male Sprague-Dawley rats were randomly divided to receive either a 70% small intestinal resection (n = 59), or a mid-small intestinal transection (n = 49). Seven d postoperatively, the animals were killed and the Na+/H+ exchanger activity of the intestinal remnants was studied by a well validated brush border membrane vesicle technique. The initial rate of Na+ uptake in the presence of an outwardly directed pH gradient and the Vmax of the amiloride-sensitive Na+ uptake were significantly increased (p < 0.01 and p < 0.001, respectively) in the resection as compared with the transection remnants and to a greater magnitude in the distal as compared with the proximal remnants. Km values were not significantly different. The amiloride-sensitive Na+ uptake in the setting of various intravesicular pH was significantly greater (p < 0.001) in the distal resection as compared with the distal transection remnants, with points of enhanced Na+/H+ exchanger activity of intravesicular pH 6.62 and 6.87, respectively. The presence and activation of the Na+/H+ exchanger's internal modifier site was confirmed by demonstrating the effect of intravesicular pH on Na+ efflux. The present study demonstrates an up-regulation of intestinal Na+/H+ exchange activity in a small-bowel resection model in the weanling rat.(ABSTRACT TRUNCATED AT 250 WORDS)

MeSH Terms (15)

Amiloride Animals Carrier Proteins Hydrogen-Ion Concentration Hyperplasia Intestine, Small In Vitro Techniques Ion Transport Kinetics Male Microvilli Rats Rats, Sprague-Dawley Sodium-Hydrogen Exchangers Up-Regulation

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