Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage.

Slichenmyer WJ, Nelson WG, Slebos RJ, Kastan MB
Cancer Res. 1993 53 (18): 4164-8

PMID: 8364909

Cell cycle checkpoints regulate progression through the cell cycle. In yeast, loss of the G2 checkpoint by mutation of the rad9 gene results in increased genetic instability as well as increased sensitivity to ionizing radiation. In contrast, comparing clonogenic survival of cells which are isogeneic except for p53 functional status, we find that loss of a G1 checkpoint in mammalian cells is not associated with increased sensitivity to the lethal effects of ionizing radiation or a topoisomerase I inhibitor, camptothecin. These results indicate that increased sensitivity to DNA-damaging agents is not necessarily a defining feature of a mammalian cell cycle checkpoint. Furthermore, in light of a recent link of p53 function to radiation-induced apoptosis in hematopoietic cells, these observations suggest that p53-dependent apoptosis is a cell type-specific phenomenon and thus predict that the biological consequences of loss of p53 function will be cell type specific.

MeSH Terms (9)

Camptothecin Cell Survival DNA Damage G1 Phase Genes, p53 Humans Mutation Radiation Tolerance Tumor Cells, Cultured

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