Gamma irradiation increases hsp-70 in Chinese hamster ovary cells.

Sierra-Rivera E, Voorhees GJ, Freeman ML
Radiat Res. 1993 135 (1): 40-5

PMID: 8327659 · DOI:10.2307/3578394

The induction of hsp-70 by gamma irradiation was studied in Chinese hamster ovary (CHO) cells. Synthesis of hsp-70 was assessed by SDS-PAGE of radiolabeled proteins while levels of hsp-70 mRNA were estimated by Northern blot analysis of total RNA. SDS-PAGE analysis indicated that the synthesis of hsp-70 was enhanced in cells exposed to 400 or 1000 Gy. Northern blot analysis of RNA from cells exposed to 1000 Gy indicated a rapid increase in mRNA capable of hybridizing to an hsp-70 oligonucleotide probe. These experiments represent the first report demonstrating that ionizing radiation can increase hsp-70 mRNA.

MeSH Terms (8)

Animals Blotting, Northern CHO Cells Cricetinae Gamma Rays Heat-Shock Proteins Nucleic Acid Hybridization RNA, Messenger

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