The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb.

Ohi R, McCollum D, Hirani B, Den Haese GJ, Zhang X, Burke JD, Turner K, Gould KL
EMBO J. 1994 13 (2): 471-83

PMID: 8313892 · PMCID: PMC394831

The Schizosaccharomyces pombe cdc5+ gene was identified in the first screen for cell division cycle mutants in this yeast. The cdc5+ gene was reported to be required for nuclear division but because of its modest elongation and leaky nature at the non-permissive temperature, it was not investigated further. Here, we report the characterization of the single allele of this gene, cdc5-120, in more detail. The mutant arrests with a 2N DNA content and a single interphase nucleus. Further genetic analyses suggest that cdc5+ gene function is essential in the G2 phase of the cell cycle. We have cloned and sequenced the cdc5+ gene. The deduced protein sequence predicts that Cdc5 is an 87 kDa protein and contains a region sharing significant homology with the DNA binding domain of the Myb family of transcription factors. Deletion mapping of the cdc5+ gene has shown that the N-terminal 232 amino acids of the protein, which contain the Myb-related region, are sufficient to complement the cdc5ts strain. A cdc5 null mutant was generated by homologous recombination. Haploid cells lacking cdc5+ are inviable, indicating that cdc5+ is an essential gene. A fusion protein consisting of bacterial glutathione S-transferase joined in-frame to the N-terminal 127 amino acids of the Cdc5 protein is able to bind to DNA cellulose at low salt concentrations. This evidence suggests that cdc5+ might encode a transcription factor whose activity is required for cell cycle progression and growth during G2.

MeSH Terms (19)

Amino Acid Sequence Base Sequence Cell Cycle Proteins Cloning, Molecular DNA, Fungal DNA-Binding Proteins DNA Replication Fungal Proteins Genes, Fungal Genetic Complementation Test Hydroxyurea Molecular Sequence Data Phenotype Proto-Oncogene Proteins Proto-Oncogene Proteins c-myb Restriction Mapping Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid

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