Novel Pseudomonas product stimulates interleukin-8 production in airway epithelial cells in vitro.

Massion PP, Inoue H, Richman-Eisenstat J, Grunberger D, Jorens PG, Housset B, Pittet JF, Wiener-Kronish JP, Nadel JA
J Clin Invest. 1994 93 (1): 26-32

PMID: 8282796 · PMCID: PMC293717 · DOI:10.1172/JCI116954

Because high concentrations of IL-8 are found in the sputum of cystic fibrosis patients, we hypothesized that Pseudomonas aeruginosa (PA) induces the production of IL-8 in airway epithelial cells and in monocytes. Therefore, we incubated the supernatant from PA culture with human transformed bronchial epithelial cells (16-HBE) or with monocytes. The culture medium of 16-HBE cells that had been incubated with PA supernatant for 6 h had chemotactic activity that was inhibited by an antibody to human IL-8. The PA supernatant induced IL-8 production by primary bronchial epithelial cells, by 16-HBE cells, and by monocytes. After incubation with PA supernatant, 16-HBE cells showed a marked increase in the levels of IL-8 gene expression. The PA product responsible for IL-8 production resisted freezing, boiling, and proteolysis. This product was not lipid extractable and was present in a 1-kD filtrate. We conclude that a small molecular mass product of PA stimulates IL-8 production by 16-HBE cells and by monocytes, and that the chemotactic activity produced by 16-HBE cells after exposure to PA is due principally to IL-8.

MeSH Terms (15)

Blotting, Northern Bronchi Cell Line Cell Line, Transformed Cells, Cultured Chemotaxis, Leukocyte Culture Media Epithelium Humans Interleukin-8 Monocytes Neutrophils Pseudomonas aeruginosa RNA, Messenger Species Specificity

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