Amino acid substitutions in the floor of the putative antigen-binding site of H-2T22 affect recognition by a gamma delta T-cell receptor.

Moriwaki S, Korn BS, Ichikawa Y, van Kaer L, Tonegawa S
Proc Natl Acad Sci U S A. 1993 90 (23): 11396-400

PMID: 8248260 · PMCID: PMC47989 · DOI:10.1073/pnas.90.23.11396

We have previously identified a self-reactive gamma delta T-cell clone (KN6) specific for the H-2T region gene product T22b. Now we have investigated by an in vitro mutagenesis analysis of the T22b gene the possibility that the interaction between the KN6 gamma delta T-cell receptor and T22b involves a peptide. The results demonstrate that mutations at the floor of the putative antigen-binding groove of T22b affect recognition by the gamma delta T-cell receptor. Furthermore, we have shown that KN6 cells react with cells that are deficient in the class I peptide transporter TAP1/TAP2. These results suggest that peptide is involved in the interaction of the KN6 T-cell receptor with T22 and that loading of T22 with the putative peptide is TAP1/TAP2-independent.

MeSH Terms (18)

Animals ATP-Binding Cassette Transporters ATP Binding Cassette Transporter, Subfamily B, Member 2 ATP Binding Cassette Transporter, Subfamily B, Member 3 Biological Transport Carrier Proteins Genes, MHC Class II H-2 Antigens Histocompatibility Antigens Class II Lymphocyte Activation Mice Mutagenesis, Site-Directed Protein Binding Protein Structure, Tertiary Receptors, Antigen, T-Cell, gamma-delta Recombinant Proteins Structure-Activity Relationship Transfection

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