Yeast Taf170 is encoded by MOT1 and exists in a TATA box-binding protein (TBP)-TBP-associated factor complex distinct from transcription factor IID.

Poon D, Campbell AM, Bai Y, Weil PA
J Biol Chem. 1994 269 (37): 23135-40

PMID: 8083216

Our characterization of the Saccharomyces cerevisiae TATA box-binding protein (TBP) has led to the identification of nine specific yeast TBP-associated factors (TAFs) ranging in size from 170 to 25 kDa. The amino acid sequence derived from a purified TAF with an apparent M(r) of 170,000 indicates that yeast Taf170 is encoded by the essential yeast gene MOT1. We describe in this report a series of experiments that demonstrate that the protein encoded by MOT1 is a bona fide yeast TAF and that Taf170 forms a separate complex with TBP distinct from the RNA polymerase II-specific multisubunit transcription factor IID TBP-TAF complex. The significance of this unique TBP-Taf170 complex regarding transcriptional regulation is discussed.

MeSH Terms (18)

Adenosine Triphosphatases Amino Acid Sequence Base Sequence Blotting, Western Cloning, Molecular DNA-Binding Proteins DNA Helicases Electrophoresis, Polyacrylamide Gel Molecular Sequence Data Oligodeoxyribonucleotides Precipitin Tests Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors TATA-Box Binding Protein TATA Box Transcription Factors Transcription Factor TFIID

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