Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver.

Chaudhary AK, Nokubo M, Reddy GR, Yeola SN, Morrow JD, Blair IA, Marnett LJ
Science. 1994 265 (5178): 1580-2

PMID: 8079172 · DOI:10.1126/science.8079172

Endogenous DNA adducts may contribute to the etiology of human genetic disease and cancer. One potential source of endogenous DNA adducts is lipid peroxidation, which generates mutagenic carbonyl compounds such as malondialdehyde. A sensitive mass spectrometric method permitted detection and quantitation of the major malondialdehyde-DNA adduct, a pyrimidopurinone derived from deoxyguanosine. DNA from disease-free human liver was found to contain 5400 adducts per cell, a frequency comparable to that of adducts formed by exogenous carcinogens.

MeSH Terms (16)

Adolescent Adult Animals Carbon Tetrachloride Deoxyguanosine DNA DNA Damage Female Gas Chromatography-Mass Spectrometry Humans Lipid Peroxidation Liver Male Malondialdehyde Rats Rats, Sprague-Dawley

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