Shuttle mutagenesis of Legionella pneumophila: identification of a gene associated with host cell cytopathicity.

Arroyo J, Hurley MC, Wolf M, McClain MS, Eisenstein BI, Engleberg NC
Infect Immun. 1994 62 (9): 4075-80

PMID: 8063428 · PMCID: PMC303072 · DOI:10.1128/IAI.62.9.4075-4080.1994

We performed shuttle mutagenesis of Legionella pneumophila. Mutants were screened for reduced cellular infectivity. Approximately 10% of the mutants had decreased cytopathicity. The DNA sequence of one locus was determined; the inferred amino acid sequence revealed homology with transport proteins including Escherichia coli TolC, Bordetella pertussis CyaE, and Alcaligenes eutrophus CzcC and CnrC.

MeSH Terms (10)

Amino Acid Sequence Bacterial Proteins Base Sequence Carrier Proteins DNA Transposable Elements Genes, Bacterial Humans Legionella pneumophila Molecular Sequence Data Mutagenesis

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