Cloning of multiple forms of goldfish vimentin: differential expression in CNS.

Glasgow E, Druger RK, Fuchs C, Levine EM, Giordano S, Schechter N
J Neurochem. 1994 63 (2): 470-81

PMID: 8035174 · DOI:10.1046/j.1471-4159.1994.63020470.x

In efforts to determine the primary structure of intermediate filament proteins in the goldfish visual pathway, we isolated clones from a retinal lambda gt11 cDNA expression library that represent goldfish vimentin. We show that there are at least two forms of goldfish vimentin, designated as vimentin alpha and vimentin beta. RNase protection assays indicate that vimentin alpha mRNA is expressed in low amounts in retina, optic nerve, and brain and in higher amounts in spinal cord. In contrast, vimentin beta mRNA is expressed in low amounts in retina, optic nerve, brain, and spinal cord and in very high amounts in eye lens. Immunohistochemical studies show that in the optic nerve, vimentin alpha is mainly restricted to blood vessels, meninges, and septa. Light staining is observed with this antibody in an astrocytic glial pattern throughout the optic nerve. Two-dimensional gel analysis shows that all of these goldfish vimentins are low abundant components of optic nerve cytoskeletal preparations.

MeSH Terms (25)

Amino Acid Sequence Animals Base Sequence Brain Carps Central Nervous System Cloning, Molecular DNA, Complementary DNA Primers Gene Expression Goldfish Immunohistochemistry Intermediate Filament Proteins Lens, Crystalline Molecular Sequence Data Optic Nerve Organ Specificity Polymerase Chain Reaction Rats Retina RNA, Messenger Sequence Homology, Amino Acid Spinal Cord Vimentin Xenopus

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