Positive selection of self- and alloreactive CD8+ T cells in Tap-1 mutant mice.

Aldrich CJ, Ljunggren HG, Van Kaer L, Ashton-Rickardt PG, Tonegawa S, Forman J
Proc Natl Acad Sci U S A. 1994 91 (14): 6525-8

PMID: 8022816 · PMCID: PMC44235 · DOI:10.1073/pnas.91.14.6525

Mice with a homozygous deletion in their Tap-1 gene (-/- mice) express very low levels of cell membrane major histocompatibility complex class I molecules and have < 1% peripheral CD8+ T cells. We show that these -/- mice but not their +/- littermates display strong primary syngeneic anti-H-2Kb and -Db-specific responses mediated by CD8+ T cells. These responses are augmented by in vivo priming. Further, -/- mice primed in vivo with H-2d alloantigens generate an anti-H-2d response which appears nearly as strong as that found in +/- littermates. Both -/- anti-H-2b and anti-H-2d T cells do not recognize target cells from Tap-1 -/- animals or Tap-2-deficient RMA-S cells. Thus, some CD8+ anti-self and alloreactive T cells can be selected in the absence of Tap proteins.

MeSH Terms (15)

Animals ATP-Binding Cassette Transporters ATP Binding Cassette Transporter, Subfamily B, Member 2 beta 2-Microglobulin Carrier Proteins Cytotoxicity, Immunologic H-2 Antigens Lymphocyte Activation Mice Mice, Inbred C57BL Mice, Mutant Strains Neoplasm Transplantation Spleen T-Lymphocytes T-Lymphocytes, Cytotoxic

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