Expression, purification, and characterization of porcine leukocyte 12-lipoxygenase produced in the methylotrophic yeast, Pichia pastoris.

Reddy RG, Yoshimoto T, Yamamoto S, Marnett LJ
Biochem Biophys Res Commun. 1994 205 (1): 381-8

PMID: 7999053 · DOI:10.1006/bbrc.1994.2676

A cDNA coding for porcine leukocyte 12-lipoxygenase was expressed intracellularly in the methylotrophic yeast Pichia pastoris under the regulatory control of the alcohol oxidase promoter. The recombinant 12-lipoxygenase contained in the yeast cell lysate was soluble, displayed the catalytic properties of the native enzyme, and was recognized by antibodies prepared against native 12-lipoxygenase derived from porcine leukocytes. The catalytically active enzyme of the 100,000 x g supernatant obtained from the yeast lysate was readily purified by immunoaffinity chromatography to near homogeneity. Porcine leukocyte 12-lipoxygenase is the first arachidonic acid oxygenase to be expressed in yeast, an easy, inexpensive, and rapid method of expressing native and site-directed mutants of recombinant proteins.

MeSH Terms (12)

Animals Arachidonate 12-Lipoxygenase Blotting, Western Chromatography, Affinity DNA, Complementary Electrophoresis, Polyacrylamide Gel Escherichia coli Leukocytes Pichia Plasmids Recombinant Proteins Swine

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