Induction of 15-lipoxygenase by interleukin-13 in human blood monocytes.

Nassar GM, Morrow JD, Roberts LJ, Lakkis FG, Badr KF
J Biol Chem. 1994 269 (44): 27631-4

PMID: 7961680

15-Lipoxygenase (15-LO) catalyzes hydroperoxidation of fatty acids, a reaction of potential relevance to inflammation, membrane remodeling, and atherosclerosis. In human leukocytes, 15-lipoxygenation of arachidonic acid produces 15-(S)-hydroxyeicosatetraenoic acid and lipoxin A4, which suppress white cell chemotaxis, adherence, and activation, and antagonize proinflammatory leukotrienes. Interleukin (IL)-13, produced by T-helper subset 2 (TH-2) lymphocytes, specifically and potently induced 15-LO gene expression and enzyme activity in human monocytes. Among other TH-2 lymphokines, this induction of 15-LO is shared by IL-4 but not by IL-10. Interferon-gamma, a product of TH-1 lymphocytes, blocked IL-13-mediated induction of 15-LO. The induction of the anti-inflammatory 15-LO pathway by IL-13 reveals a new facet of IL-13 biology that supports its role as a cytokine with potential to down-regulate inflammatory pathways. The contrasting effects of interferon-gamma and IL-13 on 15-LO induction demonstrate mechanisms by which T-lymphocyte subsets may modulate macrophage/monocyte function in inflammation or atherosclerosis.

MeSH Terms (13)

Arachidonate 15-Lipoxygenase Base Sequence DNA Primers Gene Expression Humans Hydroxyeicosatetraenoic Acids Interleukin-4 Interleukin-10 Interleukin-13 In Vitro Techniques Molecular Sequence Data Monocytes RNA, Messenger

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