Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA.

Smider V, Rathmell WK, Lieber MR, Chu G
Science. 1994 266 (5183): 288-91

PMID: 7939667 · DOI:10.1126/science.7939667

Three genetic complementation groups of rodent cells are defective for both repair of x-ray-induced double-strand breaks and V(D)J recombination. Cells from one group lack a DNA end-binding activity that is biochemically and antigenically similar to the Ku autoantigen. Transfection of complementary DNA (cDNA) that encoded the 86-kilodalton subunit of Ku rescued these mutant cells for DNA end-binding activity, x-ray resistance, and V(D)J recombination activity. These results establish a role for Ku in DNA repair and recombination. Furthermore, as a component of a DNA-dependent protein kinase, Ku may initiate a signaling pathway induced by DNA damage.

MeSH Terms (19)

Animals Antigens, Nuclear Cell Line Cell Line, Transformed Cell Survival Cricetinae DNA DNA, Complementary DNA-Binding Proteins DNA Helicases DNA Repair Gene Rearrangement Genetic Complementation Test Humans Ku Autoantigen Nuclear Proteins Radiation Tolerance Recombination, Genetic Transfection

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