Retention of the label during the conversion of [3-3H] squalene into (3S)-2,3-oxidosqualene catalyzed by mammalian squalene oxidase.

Boutaud O, Ceruti M, Cattel L, Schuber F
Biochem Biophys Res Commun. 1995 208 (1): 42-7

PMID: 7887959 · DOI:10.1006/bbrc.1995.1302

Squalene epoxidase is the only known flavoprotein that catalyzes the epoxidation of an olefin. In order to test the possibility of a catalytic non-heme metal-based mechanism, the conversion of chemically synthesized [3-3H]squalene into [3H]2,3-oxidosqualene, by partially purified pig liver squalene epoxidase, was studied. No exchange of the labeled hydrogen could be observed, ruling out a mechanism involving, e.g., an iron carbene type species at C-3.

MeSH Terms (11)

Animals Biotransformation Lanosterol Microsomes, Liver NADPH-Ferrihemoprotein Reductase Oxygenases Radioisotope Dilution Technique Squalene Squalene Monooxygenase Swine Tritium

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