Noradrenergic lesioning with an anti-dopamine beta-hydroxylase immunotoxin.

Picklo MJ, Wiley RG, Lappi DA, Robertson D
Brain Res. 1994 666 (2): 195-200

PMID: 7882029 · DOI:10.1016/0006-8993(94)90772-2

Sympathectomy has been achieved by a variety of methods but each has its limitations. These include lack of tissue specificity, incomplete lesioning, and the age range of susceptibility to the lesioning. To circumvent these drawbacks, an immunotoxin was constructed using a monoclonal antibody against the noradrenergic specific enzyme dopamine beta-hydroxylase (D beta H) coupled via a disulfide bond to saporin, a ribosomal inactivating protein. Three days after intravenous injection of the anti-D beta H immunotoxin (50 micrograms) into adult Sprague-Dawley rats, 66% of neurons in the superior cervical ganglia were chromatolytic. Superior cervical ganglia neurons were poisoned in 1 day old and 1 week old (86% of neurons) neonatal rats following subcutaneous injection of 3.75 and 15 micrograms, respectively. The anti-D beta H immunotoxin will be a useful tool in the study of the peripheral noradrenergic system in adult and neonatal animals.

MeSH Terms (17)

Animals Antibodies, Monoclonal Benzoxazines Coloring Agents Dopamine beta-Hydroxylase Ganglia, Sympathetic Immunotoxins Male N-Glycosyl Hydrolases Norepinephrine Oxazines Plant Proteins Rats Rats, Sprague-Dawley Ribosome Inactivating Proteins, Type 1 Saporins Sympathectomy, Chemical

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