Glutamate receptor RNA editing in vitro by enzymatic conversion of adenosine to inosine.

Rueter SM, Burns CM, Coode SA, Mookherjee P, Emeson RB
Science. 1995 267 (5203): 1491-4

PMID: 7878468 · DOI:10.1126/science.7878468

RNA encoding the B subunit of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) subtype of ionotropic glutamate receptor (GluR-B) undergoes a posttranscriptional modification in which a genomically encoded adenosine is represented as a guanosine in the GluR-B complementary DNA. In vitro editing of GluR-B RNA transcripts with HeLa cell nuclear extracts was found to result from an activity that converts adenosine to inosine in regions of double-stranded RNA by enzymatic base modification. This activity is consistent with that of a double-stranded RNA-specific adenosine deaminase previously described in Xenopus oocytes and widely distributed in mammalian tissues.

MeSH Terms (19)

Adenosine Animals Base Sequence Cell Line Codon Exons HeLa Cells Humans Inosine Inosine Monophosphate Mice Molecular Sequence Data Rats Receptors, AMPA Repetitive Sequences, Nucleic Acid RNA, Double-Stranded RNA Editing RNA Precursors Tumor Cells, Cultured

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