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A tissue-culture model for the study of canine vocal fold fibroblasts.

Broadley C, Gonzalez DA, Nair R, Koriwchak MJ, Ossoff RH, Davidson JM
Laryngoscope. 1995 105 (1): 23-7

PMID: 7837909 · DOI:10.1288/00005537-199501000-00008

A tissue-culture model has been developed for the study of fibroblasts from the canine vocal fold. Laryngeal tissue (lamina propria) obtained from euthanized dogs is rinsed, cut into 1-mm3 pieces, and incubated in 5% carbon dioxide at 37 degrees C. A confluent monolayer is established within several days. Detectable levels of elastin in the tissue culture supernatant are measured by an indirect enzyme-linked immunosorbent assay. Various external agents have been shown to affect elastin production. The effects of KTP laser irradiation, hydrocortisone (1.3 mumol/L), transforming growth factor-beta (10 ng/mL), and human leukocyte elastase have been measured. Thus the canine vocal fold fibroblast tissue culture is established as a model for further investigations to improve wound healing and to understand the wound-healing process following laryngeal microsurgery.

MeSH Terms (22)

Animals Carbon Dioxide Cell Movement Cell Separation Culture Media Culture Techniques Dogs Elastin Enzyme-Linked Immunosorbent Assay Fibroblasts Humans Hydrocortisone Larynx Lasers Leukocyte Elastase Microsurgery Models, Biological Pancreatic Elastase Transforming Growth Factor beta Tropoelastin Vocal Cords Wound Healing

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