Increase in positive selection of CD8+ T cells in TAP1-mutant mice by human beta 2-microglobulin transgene.

Martien van Santen H, Woolsey A, Rickardt PG, Van Kaer L, Baas EJ, Berns A, Tonegawa S, Ploegh HL
J Exp Med. 1995 181 (2): 787-92

PMID: 7836931 · PMCID: PMC2191882 · DOI:10.1084/jem.181.2.787

Mice harboring a deletion of the gene encoding the transporter associated with antigen presentation-1 (TAP1) are impaired in providing major histocompatibility complex (MHC) class I molecules with peptides of cytosolic origin and lack stable MHC class I cell surface expression. They consequently have a strongly reduced number of CD8+ T cells. To examine whether selection of CD8+ T cells is dependent on TAP-dependent peptides, we partially restored MHC class I cell surface expression in TAP1-deficient mice by introduction of human beta 2-microglobulin. We show that selection of functional CD8+ T cells can be augmented in vivo in the absence of TAP1-dependent peptides.

MeSH Terms (12)

Amino Acid Sequence Animals Antigens ATP-Binding Cassette Transporters ATP Binding Cassette Transporter, Subfamily B, Member 2 beta 2-Microglobulin Cell Differentiation Humans Mice Mice, Transgenic Molecular Sequence Data T-Lymphocytes, Regulatory

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