Serological, functional, and immunochemical characterization of a monoclonal antibody (MoAb Q2/70) to human Ia-like antigens.

Quaranta V, Indiveri F, Glassy MC, Ng A, Russo C, Molinaro GA, Pellegrino MA, Ferrone S
Hum Immunol. 1980 1 (3): 211-23

PMID: 6790480 · DOI:10.1016/0198-8859(80)90016-6

Serological and immunochemical studies showed that monoclonal antibody Q2/70 (MoAb Q2/70), produced by the hybridoma technique, is specific for human Ia-like antigens. This antibody recognizes an antigenic determinant which is different from those defining the serologic polymorphism of Ia-like antigens, and is expressed on subsets of human Ia-like molecules and on lymphoid cells from other species. MoAb Q2/70 inhibits unidirectional MLRs* between allogenic human lymphocytes, but not between murine and human lymphocytes. In ADCC* assays. MoAb Q2/70 mediates lysis of cultured human B lymphoid cells RPMI 4098, effected by murine splenocytes. The antibody is suitable to isolate immunologically functional B lymphocytes from human peripheral blood.

MeSH Terms (24)

Animals Antibodies Antibodies, Anti-Idiotypic Antibodies, Monoclonal Binding Sites, Antibody Cattle Cell Separation Complement System Proteins Cytotoxicity, Immunologic Electrophoresis, Polyacrylamide Gel Goats Guinea Pigs Haplorhini Histocompatibility Antigens Class II Humans Macromolecular Substances Mice Puromycin Rabbits Rats Rosette Formation Sheep Staphylococcal Protein A Tunicamycin

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