A comparison of t-butyldimethylsilyl and trimethylsilyl ether derivatives for the characterization of urinary metabolites of prostaglandin F2alpha by gas chromatography mass spectrometry.

Brash AR, Baillie TA
Biomed Mass Spectrom. 1978 5 (5): 346-56

PMID: 656559 · DOI:10.1002/bms.1200050506

t-Butyldimethylsilyl and trimethylsilyl ether derivatives of a series of methylated prostaglandin F2alpha metabolites have been compared with respect to their gas chromatographic and mass spectrometric properties. The t-butyldimethylsilyl derivatives had considerably higher retention indices (approximately 2.2 C units per silyl group) than their trimethylsilyl ether counterparts when analysed on the (non-polar) OV-1 stationary phase. Electron impact induced fragmentation patterns were strongly dependent upon the type of silyl ether employed and on the nature of the prostaglandin omega sidechain; the mass spectra of pairs of t-butyldimethylsilyl and trimethylsilyl ether derivatives were found to differ appreciably in several respects and to afford complementatary structural information.

MeSH Terms (12)

Animals Chemical Phenomena Chemistry Chromatography, Gas Ethers Lactones Male Mass Spectrometry Prostaglandins F Rats Silicon Trimethylsilyl Compounds

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