A new microspectrophotometric method for measuring absorbance of rat photoreceptors.

Penn JS, Williams TP
Vision Res. 1984 24 (11): 1673-6

PMID: 6533992 · DOI:10.1016/0042-6989(84)90325-0

A method has been developed for obtaining visual pigment absorbance spectra from fixed, frozen sections of the albino rat retina. The retinas are transversely sectioned at various thicknesses on a cryo-microtome and the resulting slices are examined with a photon-counting microspectrophotometer. Problems caused by the limited pigment content of small receptors are reduced and measurements can be made at well-specified locations along the retinal section. This method is both precise and accurate and permits comparisons across sections and across animals.

MeSH Terms (9)

Animals Frozen Sections Microtomy Rats Rats, Inbred Strains Retina Rhodopsin Rod Cell Outer Segment Spectrophotometry

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