Use of a cellular polyadenylation signal by viral transcripts in polyoma virus transformed cells.

Ruley HE, Lania L, Chaudry F, Fried M
Nucleic Acids Res. 1982 10 (15): 4515-24

PMID: 6290990 · PMCID: PMC321108 · DOI:10.1093/nar/10.15.4515

The DNA sequences at and around the junctions between viral and cellular DNA in the polyoma virus transformed mouse cell line, TS-A-3T3, have been determined. No common sequence specificity or structural features at the joins have been observed. The sequence indicates that the 94K truncated large T antigen found in TS-A-3T3 cells is a hybrid protein in which the carboxy-terminal 19 amino acids are encoded by adjacent host sequences. Moreover, the three early region transcripts initiated in viral sequences are also hybrid in nature and appear to utilize a host polyadenylation signal associated with the hexanucleotide, AATAAA, found 100 bp beyond a viral-host join.

MeSH Terms (13)

Amino Acid Sequence Animals Antigens, Viral Antigens, Viral, Tumor Base Sequence Cell Line Cell Transformation, Viral Cloning, Molecular DNA Restriction Enzymes Mice Poly A Polyomavirus Transcription, Genetic

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