A human Ia cytoplasmic determinant located on multiple forms of invariant chain (gamma, gamma 2, gamma 3).

Quaranta V, Majdic O, Stingl G, Liszka K, Honigsmann H, Knapp W
J Immunol. 1984 132 (4): 1900-5

PMID: 6199417

An antigenic determinant present in the cytoplasm, but not on the surface membrane of human Ia+ cells, is defined by a monoclonal antibody (VIC-Y1) and is shown by immunoprecipitation and by NEPHGE to be expressed by Ia oligomers. Immunoprecipitations of cellfree translates and of purified Ia subunits indicate that the VIC-Y1 determinant is located on the Ia gamma-(invariant) chain, as well as on two other related molecules, provisionally termed gamma 2 and gamma 3. Within our experimental conditions, the three forms of gamma-chains co-precipitate exclusively with Ia oligomers. As detected by VIC-Y1 and in the limits of our assays, gamma-chains could not be found at the cell surface; their tissue distribution, determined by cytoplasmic indirect immunofluorescence with VIC-Y1, closely resembles that of Ia antigens, with the possible exception of acute lymphatic leukemia cells (Ia+, gamma-chain-).

MeSH Terms (14)

Animals Antibodies, Monoclonal B-Lymphocytes Cytoplasm Electrophoresis, Polyacrylamide Gel Epitopes Fluorescent Antibody Technique Genes, MHC Class II Histocompatibility Antigens Class II Humans Mice Mice, Inbred BALB C Polymorphism, Genetic Rabbits

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