Prolactin-mediated transcriptional and post-transcriptional control of casein gene expression.

Guyette WA, Matusik RJ, Rosen JM
Cell. 1979 17 (4): 1013-23

PMID: 487427 · DOI:10.1016/0092-8674(79)90340-4

The mechanism by which prolactin, a peptide hormone, regulates casein gene expression has been studied in mammary gland organ culture. After prolactin addition, a 2-4 fold increase in the rate of casein mRNA transcription was observed within 1 hr and maintained for at least 24 hr. This increased rate of transcription is not sufficient to account for the mass accumulation of casein mRNA. The half-life of casein mRNA is also increased 17-25 fold in the presence of prolactin. This change in casein mRNA half-life, coupled with a 2-4 fold increase in the rate of transcription, can account for the normal accumulation of casein mRNA observed after prolactin addition. This hormone-induced change in casein mRNA half-life appeared to be selective, since prolactin was found to exert only a slight effect (1-4 fold) on the half-life of poly(A) RNA determined under identical pulse-chase conditions. The hormonal regulation of casein gene expression thus does not app-ar to be an "all or none" process occurring only at the transcriptional or post-transcriptional levels, but rather may involve a coordinated response at several levels to permit the efficient expression of specialized differentiated functions.

MeSH Terms (12)

Animals Caseins Female Genes Mammary Glands, Animal Organ Culture Techniques Pregnancy Prolactin Rats RNA, Messenger Time Factors Transcription, Genetic

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