Mechanism of epinephrine's glycogenolytic effect in isolated canine hepatocytes.

Steiner KE, Stevenson RW, Green DR, Cherrington AD
Metabolism. 1985 34 (11): 1020-3

PMID: 4058306 · DOI:10.1016/0026-0495(85)90073-3

Epinephrine (10(-7) mol/L) addition to isolated canine hepatocytes activates glycogen phosphorylase from 12.3 +/- 0.4 to 28.6 +/- 2.6 U/g and glucose output from 42 +/- 3 to 170 +/- 24 nmol/mg/h. Preincubation of hepatocytes with propranolol (2 X 10(-5) mol/L) caused a 73% inhibition of phosphorylase activation and a 77% inhibition of the stimulation of glucose output by epinephrine. Phentolamine (2 X 10(-5) mol/L) on the other hand, caused a 16% inhibition of phosphorylase activation and a 27% inhibition of the stimulation of glucose output by epinephrine. These results were unaffected by the sex of the animal. In the dog the glycogenolytic effects of epinephrine appear to be mediated primarily by a beta-adrenergic mechanism.

MeSH Terms (13)

Animals Dogs Enzyme Activation Epinephrine Female Glucose In Vitro Techniques Liver Male Phentolamine Phosphorylases Propranolol Sex Factors

Connections (1)

This publication is referenced by other Labnodes entities:

Links