The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria.

Cook RJ, Misono KS, Wagner C
J Biol Chem. 1985 260 (24): 12998-3002

PMID: 4055729

The flavoenzymes dimethylglycine dehydrogenase (EC and sarcosine dehydrogenase (EC contain covalently bound FAD linked via the 8 alpha-position of the isoalloxazine ring to the imidazole N(3) of a histidine residue (Cook, R. J., Misono, K. S., and Wagner, C. (1984) J. Biol. Chem. 259, 12475-12480). The flavin-peptides from tryptic digests of these two enzymes have been isolated and sequenced. Automated sequence analysis showed that the flavin-peptide from dimethylglycine dehydrogenase contained 25 amino acid residues in the following sequence: Ser-Glu-Leu-Thr-Ala-Gly-Ser- Thr-Trp-His(flavin)-Ala-Ala-Gly-Leu-Thr-Thr-Tyr-Phe-His-Pro-Gly-Ile-A sn-Leu-Lys. The sequence determined for the flavin-peptide from sarcosine dehydrogenase contained 14 amino acid residues Leu-Thr-Ser-Gly-Thr-Thr-Trp-His(flavin)-Thr-Ala-Gly-Leu-Gly-Arg.

MeSH Terms (14)

Amino Acid Sequence Animals Dimethylglycine Dehydrogenase Flavin-Adenine Dinucleotide Male Mitochondria, Liver Mitochondrial Proteins Oxidoreductases, N-Demethylating Peptide Fragments Rats Rats, Inbred Strains Sarcosine Dehydrogenase Spectrophotometry Trypsin

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