Expression at the cell surface of biologically active fusion and hemagglutinin/neuraminidase proteins of the paramyxovirus simian virus 5 from cloned cDNA.

Paterson RG, Hiebert SW, Lamb RA
Proc Natl Acad Sci U S A. 1985 82 (22): 7520-4

PMID: 3865176 · PMCID: PMC390848 · DOI:10.1073/pnas.82.22.7520

cDNAs encoding the mRNAs for the fusion protein (F) and the hemagglutinin/neuraminidase protein (HN) of the paramyxovirus simian virus 5 have been inserted into a eukaryotic expression vector under the control of the simian virus 40 late promoter. The F and HN proteins synthesized in recombinant infected cells are indistinguishable in terms of electrophoretic mobility and glycosylation from the proteins synthesized in simian virus 5-infected cells. In addition, the expressed F and HN proteins have been shown to be anchored in the plasma membrane in a biologically active form by indirect live cell immunofluorescence, the F-mediated formation of syncytia, and the ability of HN to cause the hemadsorption of erythrocytes to the infected cell surface.

MeSH Terms (8)

Animals Cattle DNA Hemagglutinins, Viral Neuraminidase Paramyxoviridae Viral Envelope Proteins Viral Fusion Proteins

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