Monocyte-T lymphocyte interaction for regulation of insulin receptors of the activated T lymphocyte.

Helderman JH, Ayuso R, Rosenstock J, Raskin P
J Clin Invest. 1987 79 (2): 566-71

PMID: 3543055 · PMCID: PMC424128 · DOI:10.1172/JCI112848

During activation the lymphocyte attains functional insulin receptors with precise regulation, a consequence of insulin concentration manipulations. These studies test the hypothesis that insulin receptor (+) monocytes monitor insulin concentrations, so instructing the T lymphocytes. Monocyte-enriched populations were incubated with insulin (0-10(-6) M) followed by co-culture with T lymphocytes and an activating stimulus. A dose-related fall in T lymphocyte insulin receptor binding was observed that was specific for the monocyte as the signalling cell and for insulin as the signal received. Monocytes from normal volunteers during a euglycemic, hyperinsulinemic clamp were cultured with T lymphocytes and an activating stimulus. A decline in specific insulin receptor binding on T lymphocytes was observed, which Scatchard analysis demonstrated to be a consequence of reduction in receptor numbers. These studies demonstrate that the receptor (+) monocyte perceives the concentration of insulin and passes this information to T lymphocytes regulating the number of activation-induced insulin receptors. The interplay between the monocyte and T lymphocyte parallels the interaction of these cell types for recognition of antigen.

MeSH Terms (13)

Animals Cells, Cultured Fasting Humans Insulin In Vitro Techniques Lymphocyte Activation Male Monocytes Rats Receptor, Insulin Spleen T-Lymphocytes

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