Expression of the second calcitonin/calcitonin gene-related peptide gene in Ewing sarcoma cell lines.

Höppener JW, Steenbergh PH, Slebos RJ, Visser A, Lips CJ, Jansz HS, Bechet JM, Lenoir GM, Born W, Haller-Brem S
J Clin Endocrinol Metab. 1987 64 (4): 809-17

PMID: 3493259 · DOI:10.1210/jcem-64-4-809

The existence of two closely related calcitonin (CT)/CT gene-related peptide (CGRP) genes has been recognized in rat and man. In man, expression of the CALC-I gene produces CT and/or CGRP-I mRNA, whereas CGRP-II mRNA is transcribed from the CALC-II gene. Until recently, expression of the CALC-II gene had been detected only in a metastasis of medullary thyroid carcinoma. In this study, expression of the CALC-II gene was demonstrated by Northern blot hybridization analysis in four of six cell lines established from different Ewing sarcomas, a malignant neoplasm of bone. Expression of the CALC-I gene was not detected in any of the six cell lines. A presumed large mol wt immunoreactive precursor of CGRP-II and small amounts of mature CGRP-II, but no CT, were found in medium from IARC/EW 1 cells. Nucleotide sequence analysis of cloned cDNA from this cell line confirmed the production of CGRP-II mRNA. CALC-II gene expression in Ewing sarcoma might be useful for studies concerning regulation of gene expression in the CALC gene family and possibly for tumor classification.

MeSH Terms (9)

Base Sequence Calcitonin Calcitonin Gene-Related Peptide Cell Line Genes Neuropeptides Nucleic Acid Hybridization RNA, Messenger Sarcoma, Ewing

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