Assessment of interference by heparin cofactor II in the DuPont aca antithrombin-III assay.

Hortin GL, Tollefsen DM, Santoro SA
Am J Clin Pathol. 1988 89 (4): 515-7

PMID: 3354504 · DOI:10.1093/ajcp/89.4.515

Hereditary deficiency of antithrombin-III (AT-III), the major heparin cofactor in human plasma, is a well-established cause of recurrent venous thrombosis. Cross-reactivity of heparin cofactor II (HC II) in assays of AT-III may, in some cases, interfere with the ability to diagnose hereditary deficiency of AT-III. For that reason, we have evaluated the interference by HC II in the new DuPont aca antithrombin assay. Response of the assay to purified AT-III and HC II was compared. Inhibition of the bovine thrombin in the assay was sixfold less per molecule of HC II than of AT-III. This level of selectivity should be adequate to prevent misdiagnosis of patients. Analysis of patient samples showed close correlation (r = 0.91) of values from the automated aca assay with those of a manual assay (Coatest antithrombin, Helena Laboratories).

MeSH Terms (9)

Antithrombin III Antithrombin III Deficiency Antithrombins Cross Reactions Diagnostic Errors Glycoproteins Heparin Cofactor II Humans Reagent Kits, Diagnostic

Connections (2)

This publication is referenced by other Labnodes entities:

Links