Assessment of interference by heparin cofactor II in the DuPont aca antithrombin-III assay.

Hortin GL, Tollefsen DM, Santoro SA
Am J Clin Pathol. 1988 89 (4): 515-7

PMID: 3354504 · DOI:10.1093/ajcp/89.4.515

Hereditary deficiency of antithrombin-III (AT-III), the major heparin cofactor in human plasma, is a well-established cause of recurrent venous thrombosis. Cross-reactivity of heparin cofactor II (HC II) in assays of AT-III may, in some cases, interfere with the ability to diagnose hereditary deficiency of AT-III. For that reason, we have evaluated the interference by HC II in the new DuPont aca antithrombin assay. Response of the assay to purified AT-III and HC II was compared. Inhibition of the bovine thrombin in the assay was sixfold less per molecule of HC II than of AT-III. This level of selectivity should be adequate to prevent misdiagnosis of patients. Analysis of patient samples showed close correlation (r = 0.91) of values from the automated aca assay with those of a manual assay (Coatest antithrombin, Helena Laboratories).

MeSH Terms (9)

Antithrombin III Antithrombin III Deficiency Antithrombins Cross Reactions Diagnostic Errors Glycoproteins Heparin Cofactor II Humans Reagent Kits, Diagnostic

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