A non-AUG translational initiation in c-myc exon 1 generates an N-terminally distinct protein whose synthesis is disrupted in Burkitt's lymphomas.

Hann SR, King MW, Bentley DL, Anderson CW, Eisenman RN
Cell. 1988 52 (2): 185-95

PMID: 3277717 · DOI:10.1016/0092-8674(88)90507-7

The c-myc gene comprises three exons with a single large AUG-initiated open reading frame extending from exon 2 through exon 3. Exon 1 lacks any AUG codons. Cells from a wide range of species produce two c-myc proteins that, while highly related, do not appear to arise from posttranslational interconversion. To understand the origin of the two proteins, we mapped them and analyzed the in vitro protein-coding capacity of c-myc cDNAs. Our findings show that the two proteins are derived from alternative translational initiations at the exon 2 AUG and at a non-AUG codon near the 3' end of exon 1, resulting in the production of proteins with distinct N termini. In Burkitt's lymphomas, the removal or specific mutation of exon 1 in c-myc translocations correlates with suppression of synthesis of the larger protein, and thus may contribute to the oncogenic activation of c-myc.

MeSH Terms (19)

Animals Biological Evolution Burkitt Lymphoma Cell Line Chickens Codon DNA, Recombinant Exons Humans Mice Molecular Weight Mutation Peptide Chain Initiation, Translational Peptide Fragments Protein Biosynthesis Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc RNA, Messenger Xenopus laevis

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