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Hormonal regulation of rat Leydig cell cytochrome P-45017 alpha mRNA levels and characterization of a partial length rat P-45017 alpha cDNA.

Nishihara M, Winters CA, Buzko E, Waterman MR, Dufau ML
Biochem Biophys Res Commun. 1988 154 (1): 151-8

PMID: 3260774 · DOI:10.1016/0006-291x(88)90663-8

We have isolated and characterized a P-45017 alpha cDNA fragment from a rat testis library. The partial length rat P-45017 alpha cDNA (1Kb) has high overall nucleotide and deduced amino acid similarity with human and bovine P-45017 alpha cDNA's and contains the conserved tridecapeptide and heme regions, the termination codon and polyadenylation site. Using this rat testis cDNA probe we measured P-45017 alpha mRNA levels of rat Leydig cells from animals treated with hCG. Temporal studies with a low hCG dose showed an early increase in mRNA levels returning to control values at later times, while a higher desensitizing dose caused a marked reduction in the mRNA (24 h) and a small recovery at 48 h. Fetal rat Leydig cells maintained in the presence of LH treated with estradiol showed a 70% decrease in P-45017 alpha mRNA levels and testosterone production followed closely the changes in P-45017 alpha mRNA. These studies suggest that gonadotropin stimulation and desensitization of P-45017 alpha dependent enzymes in the adult rat testis as well as estradiol induced desensitization in fetal Leydig cells are related to levels P-45017 alpha mRNA.

MeSH Terms (17)

Amino Acid Sequence Animals Base Sequence Chorionic Gonadotropin DNA Genes Kinetics Leydig Cells Luteinizing Hormone Male Molecular Sequence Data Nucleic Acid Hybridization Rats Reference Values RNA, Messenger Steroid 17-alpha-Hydroxylase Steroid Hydroxylases

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