Active site selective labeling of serine proteases with spectroscopic probes using thioester peptide chloromethyl ketones: demonstration of thrombin labeling using N alpha-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CH2Cl.

Bock PE
Biochemistry. 1988 27 (17): 6633-9

PMID: 3219359 · DOI:10.1021/bi00417a063

MeSH Terms (10)

Amino Acid Chloromethyl Ketones Binding Sites Chromatography, High Pressure Liquid Humans Indicators and Reagents Kinetics Magnetic Resonance Spectroscopy Protein Binding Serine Endopeptidases Thrombin

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