Development of a novel murine model of lymphatic metastasis.

Banan B, Beckstead JA, Dunavant LE, Sohn Y, Adcock JM, Nomura S, Abumrad N, Goldenring JR, Fingleton B
Clin Exp Metastasis. 2020 37 (2): 247-255

PMID: 32052231 · DOI:10.1007/s10585-020-10025-3

Current laboratory models of lymphatic metastasis generally require either genetically modified animals or are technically challenging. Herein, we have developed a robust protocol for the induction of intralymphatic metastasis in wild-type mice with reproducible outcomes. To determine an optimal injection quantity and timeline for tumorigenesis, C57Bl/6 mice were injected directly into the mesenteric lymph duct (MLD) with varying numbers of syngeneic murine colon cancer cells (MC38) or gastric cancer cells (YTN16) expressing GFP/luciferase and monitored over 2-4 weeks. Tumor growth was tracked via whole-animal in vivo bioluminescence imaging (IVIS). Our data indicate that the injection of tumor cells into the MLD is a viable model for lymphatic metastasis as necropsies revealed large tumor burdens and metastasis in regional lymph nodes. This protocol enables a closer study of the role of lymphatics in cancer metastasis and opens a window for the development of novel approaches for treatment of metastatic diseases.

MeSH Terms (19)

Animals Cell Line, Tumor Colonic Neoplasms Disease Models, Animal Female Green Fluorescent Proteins Humans Luciferases Luminescent Measurements Lymphatic Metastasis Lymphatic Vessels Lymph Nodes Male Mesentery Mice Mice, Inbred C57BL Stomach Neoplasms Tomography, Optical Tumor Burden

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