Effects of ATP depletion on the mechanism of hexose transport in intact human erythrocytes.

May JM
FEBS Lett. 1988 241 (1-2): 188-90

PMID: 3143605 · DOI:10.1016/0014-5793(88)81058-5

Depletion of ATP is known to inhibit glucose transport in human erythrocytes, but the kinetic mechanism of this effect is controversial. Selective ATP depletion of human erythrocytes by 10 micrograms/ml A23187 in the presence of extracellular calcium inhibited 3-O-methylglucose influx noncompetitively and efflux competitively. ATP depletion also decreased the ability of either equilibrated 3-O-methylglucose or extracellular maltose to inhibit cytochalasin B binding in intact cells, whereas neither total high-affinity cytochalasin B binding nor its Kd was affected. Under the one-site model of hexose transport these data indicate that ATP depletion decreases both the affinity of the inward-facing glucose carrier for substrate and its ability to reorient outwardly in intact cells.

MeSH Terms (11)

3-O-Methylglucose Adenosine Triphosphate Calcimycin Cytochalasin B Erythrocytes Humans In Vitro Techniques Kinetics Maltose Methylglucosides Monosaccharide Transport Proteins

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