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Lipoxygenase catalyzed oxygenation of hydroxy fatty acids to lipoxins.

Kühn H, Brash AR, Wiesner R, Alder L
Adv Exp Med Biol. 1988 229: 39-49

PMID: 3138902 · DOI:10.1007/978-1-4757-0937-7_4

The pure lipoxygenases from rabbit reticulocytes and soybeans convert a variety of substrates (arachidonic acid, 15-HPETE, 15-HETE, 5-HETE, various DiHETE isomers) to trihydroxy eicosanoids containing a conjugated tetraene system (lipoxins). In general, the methyl esters are better substrates for lipoxin formation than are the free acids. Lipoxygenase inhibitors (5,8,11,14-eicosatetraynoic acid, nordihydroguaiaretic acid) strongly inhibit the lipoxin formation. The complete stereochemistry of the lipoxin B formed from 15S-HETE methyl ester has been established by co-chromatography with authentic standards on various types of HPLC columns, by GC/MS analysis, by gas liquid chromatography of the ozonolysis fragments of the menthoxy carbonyl derivatives and 1H-NMR studies. The molar absorption coefficient of the conjugated tetraenes was measured as epsilon 301 = 53,000. The lipoxins formed from 15-HETE and various DiHETE isomers are formed exclusively via the oxygenation pathway as shown by experiments under an 17O2 atmosphere and/or by anaerobic incubations. Our results indicate that lipoxins can be synthesized via lipoxygenase-catalyzed sequential oxygenation of polyenoic fatty acids and their hydro(pero)xy derivatives.

MeSH Terms (12)

Animals Esters Hydroxy Acids Hydroxyeicosatetraenoic Acids Lipoxins Lipoxygenase Oxidation-Reduction Rabbits Reticulocytes Soybeans Spectrophotometry, Ultraviolet Substrate Specificity

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