Lipoxin synthesis by arachidonate 12-lipoxygenase purified from porcine leukocytes.

Ueda N, Yokoyama C, Yamamoto S, Fitzsimmons BJ, Rokach J, Oates JA, Brash AR
Biochem Biophys Res Commun. 1987 149 (3): 1063-9

PMID: 3122743 · DOI:10.1016/0006-291x(87)90516-x

Arachidonate 12-lipoxygenase purified from porcine leukocytes shows 14R-oxygenase and 14, 15-leukotriene A synthase activities with 15-hydroperoxy-arachidonic acid as substrate. The enzyme transformed 5, 15-dihydroperoxy-arachidonic acid to several compounds with a conjugated tetraene. A major product was identified as 5S, 14R, 15S-trihydroperoxy-6, 10, 12-trans-8-cis-eicosatetraenoic acid, which was reduced to 5S, 14R, 15S-8-cis-lipoxin B. A requirement of molecular oxygen and the results of H2(18)O experiments suggested that formation of the latter compound was attributed mostly to the 14R-oxygenase activity of the enzyme. There were several other minor products identified as lipoxin A and B isomers. They were produced presumably by hydrolysis of 14, 15-epoxy compound formed by the leukotriene A synthase activity of 12-lipoxygenase.

MeSH Terms (12)

Animals Arachidonate 12-Lipoxygenase Arachidonate Lipoxygenases Arachidonic Acid Arachidonic Acids Hydroxyeicosatetraenoic Acids Leukocytes Leukotrienes Lipid Peroxides Lipoxins Oxygen Swine

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