Myocardial differentiation is dependent upon endocardial signaling during early cardiogenesis .

Saint-Jean L, Barkas N, Harmelink C, Tompkins KL, Oakey RJ, Baldwin HS
Development. 2019 146 (9)

PMID: 31023876 · PMCID: PMC6526719 · DOI:10.1242/dev.172619

The endocardium interacts with the myocardium to promote proliferation and morphogenesis during the later stages of heart development. However, the role of the endocardium in early cardiac ontogeny remains under-explored. Given the shared origin, subsequent juxtaposition, and essential cell-cell interactions of endocardial and myocardial cells throughout heart development, we hypothesized that paracrine signaling from the endocardium to the myocardium is crucial for initiating early differentiation of myocardial cells. To test this, we generated an , endocardial-specific ablation model using the diphtheria toxin receptor under the regulatory elements of the genomic locus (). Early treatment of mouse embryoid bodies with diphtheria toxin efficiently ablated endocardial cells, which significantly attenuated the percentage of beating EBs in culture and expression of early and late myocardial differentiation markers. The addition of Bmp2 during endocardial ablation partially rescued myocyte differentiation, maturation and function. Therefore, we conclude that early stages of myocardial differentiation rely on endocardial paracrine signaling mediated in part by Bmp2. Our findings provide novel insight into early endocardial-myocardial interactions that can be explored to promote early myocardial development and growth.

© 2019. Published by The Company of Biologists Ltd.

MeSH Terms (14)

Animals Cell Differentiation Endocardium Female Flow Cytometry Male Mice Mice, Inbred C57BL Microscopy, Fluorescence Myocardium NFATC Transcription Factors Organogenesis Reverse Transcriptase Polymerase Chain Reaction Signal Transduction

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