Generation of MLC-2v-tdTomato knock-in reporter mouse line.

Zhang Z, Nam YJ
Genesis. 2018 56 (10): e23256

PMID: 30307112 · PMCID: PMC6294655 · DOI:10.1002/dvg.23256

MLC-2v is a myosin light chain regulatory protein which is specifically expressed in ventricular cardiomyocytes and slow twitch skeletal muscle cells. MLC-2v plays critical roles in ventricular maturation during heart development. Mice lacking MLC-2v are embryonic lethal due to heart failure associated with abnormal myofibrillar organization of ventricular cardiomyocytes. To study the development of ventricular cardiac muscle and slow twitch skeletal muscle, we generated a new MLC-2v reporter mouse line by knocking-in a tdTomato reporter cassette into 3' UTR of the MLC-2v gene without disrupting the endogenous gene. Our results demonstrated specific MLC-2v-tdTomato knock-in reporter expression in ventricular cardiomyocytes and slow twitch muscle during myogenesis, precisely recapitulating the spatiotemporal expression pattern of endogenous MLC-2v. No tdTomato expression was observed in the atria, fast twitch muscle or other organs throughout development into adulthood. Isolated neonatal and adult ventricular cardiomyocytes uniformly express tdTomato. Taken together, MLC-2v-tdTomato knock-in reporter mouse model described in this article will serve as a valuable tool to study cardiac chamber and skeletal muscle specification during development and regeneration by overcoming the pitfalls of transgenic strategies.

© 2018 Wiley Periodicals, Inc.

MeSH Terms (9)

Animals Gene Knock-In Techniques Genes, Reporter Lycopersicon esculentum Mice Mice, Inbred C57BL Mice, Inbred Strains Mice, Transgenic Myosin Light Chains

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