1H homonuclear editing of rat brain using semiselective pulses.

Hetherington HP, Avison MJ, Shulman RG
Proc Natl Acad Sci U S A. 1985 82 (10): 3115-8

PMID: 2987910 · PMCID: PMC397725 · DOI:10.1073/pnas.82.10.3115

We have used a semiselective Hahn spin-echo sequence of the form (1331)-tau-(2662)-tau-AQ, delivered by a surface coil to obtain high-resolution 1H NMR spectra from the brains of intact dead rats. This sequence gave suppression of the tissue water resonance by a factor of 80,000 when tau = 68 ms. Delivery of a frequency-selective Dante pulse train to the alpha-CH resonance of lactate at 4.11 ppm, simultaneously with the 2662 refocusing pulse, altered the j-modulation in the spin-coupled beta-CH3 protons. Subtraction of this spectrum from one in which the Dante was ineffective gave an edited spectrum containing only the beta-CH3 resonance of lactate at 1.31 ppm. When the position of the Dante was shifted to 3.78 ppm to selectively invert the alpha-CH protons of alanine, an edited spectrum of alanine was obtained.

MeSH Terms (8)

Alanine Animals Brain Chemistry Lactates Magnetic Resonance Spectroscopy Protons Rats Water

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