Defining the interaction of the protease CpaA with its type II secretion chaperone CpaB and its contribution to virulence in species.

Kinsella RL, Lopez J, Palmer LD, Salinas ND, Skaar EP, Tolia NH, Feldman MF
J Biol Chem. 2017 292 (48): 19628-19638

PMID: 28982978 · PMCID: PMC5712607 · DOI:10.1074/jbc.M117.808394

, , and are a frequent cause of multidrug-resistant, healthcare-associated infections. Our previous work demonstrated that M2 possesses a functional type II secretion system (T2SS) that is required for full virulence. Further, we identified the metallo-endopeptidase CpaA, which has been shown previously to cleave human Factor V and deregulate blood coagulation, as the most abundant type II secreted effector protein. We also demonstrated that its secretion is dependent on CpaB, a membrane-bound chaperone. In this study, we show that CpaA expression and secretion are conserved across several medically relevant species. Additionally, we demonstrate that deletion of results in attenuation of M2 virulence in moth and mouse models. The virulence defects resulting from the deletion of were comparable with those observed upon abrogation of T2SS activity. The virulence defects resulting from the deletion of are comparable with those observed upon abrogation of T2SS activity. We also show that CpaA and CpaB strongly interact, forming a complex in a 1:1 ratio. Interestingly, deletion of the N-terminal transmembrane domain of CpaB results in robust secretion of CpaA and CpaB, indicating that the transmembrane domain is dispensable for CpaA secretion and likely functions to retain CpaB inside the cell. Limited proteolysis of spheroplasts revealed that the C-terminal domain of CpaB is exposed to the periplasm, suggesting that this is the site where CpaA and CpaB interact Last, we show that CpaB does not abolish the proteolytic activity of CpaA against human Factor V. We conclude that CpaA is, to the best of our knowledge, the first characterized, virulence factor secreted by species.

© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

MeSH Terms (12)

Acinetobacter Animals Factor V Larva Mice Mice, Inbred C57BL Molecular Chaperones Peptide Hydrolases Protein Binding Proteolysis Spleen Virulence

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